Based on the noise source, considering that the fluctuations of incident photons, dark current and biased signal satisfy the Poisson distribution, the corrected signal-to-noise-ratio (SNR) relation is obtained. On condition that the gain, readout rate and cooling temperature of CCD are set experimentally to be 3, 100kHz and −25°C respectively, the photon transfer curve is obtained. Meanwhile, by setting different experimental conditions,the effect of the dark current, readout rate and the system gain on the SNR of the scientific grade optical CCD is discussed respectively. The experimental results are in good agreement with the theory, which provides the good experimental basis for design of the scientific grade optical streak camera and building of the automated laboratory platform used for weak light calibration.